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Rabbit anti-PTP1B (pS50) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PTP1B (pS50)

CAS Number

9007-83-4

Synonyms

PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B

Host

Rabbit

Reactivity

H, M, R, C, D, Mk, P, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PTP1B (pS50) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of PTP1B (pS50) expression in COLO205 (A), human muscle (B), mouse heart (C) whole cell lysates.

IHC description

Immunohistochemical analysis of PTP1B (pS50) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5770

Gene ID (Mouse)

19246

Gene ID (Rat)

24697

SwissProt (Human)

P18031

SwissProt (Mouse)

P35821

SwissProt (Rat)

P20417

Available Sizes

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