Rabbit anti-PTEN Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PTEN
Synonyms
MMAC1; TEP1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
Host
Rabbit
Reactivity
H, M, R, D, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTEN. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PTEN expression in HT29 (A), NIH3T3 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of PTEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5728
Gene ID (Mouse)
19211
SwissProt (Human)
P60484
SwissProt (Mouse)
O08586
CAS Number
9007-83-4
Available Sizes
Curated Selection
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