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Rabbit anti-PSMC4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PSMC4

CAS Number

9007-83-4

Synonyms

MIP224; TBP7; 26S protease regulatory subunit 6B; 26S proteasome AAA-ATPase subunit RPT3; MB67-interacting protein; MIP224; Proteasome 26S subunit ATPase 4; Tat-binding protein 7; TBP-7

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PSMC4

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of PSMC4 expression in HepG2 (A), A549 (B), Hela (C), Jurkat (D), mouse liver (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PSMC4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5704

Gene ID (Mouse)

23996

Gene ID (Rat)

117262

SwissProt (Human)

P43686

SwissProt (Mouse)

P54775

SwissProt (Rat)

Q63570

Available Sizes

Frequently Asked Questions

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