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Rabbit anti-PSMB4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PSMB4

CAS Number

9007-83-4

Synonyms

PROS26; Proteasome subunit beta type-4; 26 kDa prosomal protein; HsBPROS26; PROS-26; Macropain beta chain; Multicatalytic endopeptidase complex beta chain; Proteasome beta chain; Proteasome chain 3; HsN3

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PSMB4

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of PSMB4 expression in MCF7 (A), SKOV3 (B), mouse thymus (C), mouse liver (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PSMB4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5692

Gene ID (Mouse)

19172

Gene ID (Rat)

58854

SwissProt (Human)

P28070

SwissProt (Mouse)

P99026

SwissProt (Rat)

P34067

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