Rabbit anti-PRKAR2B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PRKAR2B
Synonyms
CAMP-dependent protein kinase type II-beta regulatory subunit
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRKAR2B. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PRKAR2B expression in HeLa (A), SHSY5Y (B), NIH3T3 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of PRKAR2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5577
Gene ID (Mouse)
19088
Gene ID (Rat)
24679
SwissProt (Human)
P31323
SwissProt (Mouse)
P31324
SwissProt (Rat)
P12369
CAS Number
9007-83-4
Available Sizes
Curated Selection
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