Rabbit anti-PPP3R1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PPP3R1

CAS Number

9007-83-4

Synonyms

CNA2; CNB; Calcineurin subunit B type 1; Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 3 regulatory subunit B alpha isoform 1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PPP3R1

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IP (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of PPP3R1 expression in SW480 (A), MCF7 (B), mouse brain (C), rat testis (D) whole cell lysates.

IHC description

Immunohistochemical analysis of PPP3R1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.