Rabbit anti-PP2C-alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PP2C-alpha
Synonyms
PPPM1A; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human PP2C-alpha
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PP2C-alpha expression in Hela (A), HepG2 (B), mouse liver (C), rat brain (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PP2C-alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5494
Gene ID (Mouse)
19042
Gene ID (Rat)
24666
SwissProt (Human)
P35813
SwissProt (Mouse)
P49443
SwissProt (Rat)
P20650
CAS Number
9007-83-4
Available Sizes
Curated Selection
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