Rabbit anti-POLR3A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to POLR3A
Synonyms
DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III subunit C160
Host
Rabbit
Reactivity
H, M, R, B, C, D, Mk, P, Rb, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human POLR3A. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of POLR3A expression in K562 (A), MCF7 (B), COS7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of POLR3A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
11128
SwissProt (Human)
O14802
CAS Number
9007-83-4
Available Sizes
Curated Selection
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