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Rabbit anti-POLR2D Antibody

Product Specifications

Background

Rabbit polyclonal antibody to POLR2D

CAS Number

9007-83-4

Synonyms

DNA-directed RNA polymerase II subunit RPB4; RNA polymerase II subunit B4; DNA-directed RNA polymerase II subunit D; RNA polymerase II 16 kDa subunit; RPB16

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human POLR2D

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of POLR2D expression in MCF7 (A), SW620 (B), HepG2 (C), mouse spleen (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of POLR2D staining in SW620 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5433

Gene ID (Mouse)

69241

SwissProt (Human)

O15514

SwissProt (Mouse)

Q9D7M8

Available Sizes

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