Rabbit anti-PNKP Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PNKP
Synonyms
Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase
Host
Rabbit
Reactivity
H
Immunogen
Recombinant full length protein of human PNKP
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PNKP expression in A549 (A), HepG2 (B), mouse skeletal muscle (C), rat liver (D) whole cell lysates.
IHC description
Immunohistochemical analysis of PNKP staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
11284
SwissProt (Human)
Q96T60
CAS Number
9007-83-4
Available Sizes
Curated Selection
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