What is the catalog number for Rabbit anti-PKR Antibody?

The catalog number for Rabbit anti-PKR Antibody is YLD6234.

What sizes are available for Rabbit anti-PKR Antibody?

Rabbit anti-PKR Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-PKR Antibody come in?

Rabbit anti-PKR Antibody is available in 2 different pack sizes.

Is Rabbit anti-PKR Antibody in stock?

Rabbit anti-PKR Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-PKR Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PKR

CAS Number

9007-83-4

Synonyms

PKR; PRKR; Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2; p68 kinase

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKR. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of PKR expression in HeLa (A), MCF7 (B), Caco2 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of PKR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of PKR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.