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Rabbit anti-PITX1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PITX1

Synonyms

BFT; PTX1; Pituitary homeobox 1; Hindlimb-expressed homeobox protein backfoot; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1

Host

Rabbit

Reactivity

H, M, R, C

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PITX1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PITX1 expression in Jurkat (A), NIH3T3 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of PITX1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5307

Gene ID (Mouse)

18740

Gene ID (Rat)

113983

SwissProt (Human)

P78337

SwissProt (Mouse)

P70314

SwissProt (Rat)

Q99NA7

CAS Number

9007-83-4

Available Sizes

Curated Selection

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