Rabbit anti-PHPT1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PHPT1
CAS Number
9007-83-4
Synonyms
PHP14; 14 kDa phosphohistidine phosphatase; Phosphohistidine phosphatase 1; Protein janus-A homolog
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human PHPT1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of PHPT1 expression in A549 (A), THP1 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PHPT1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
29085
Gene ID (Mouse)
75454
SwissProt (Human)
Q9NRX4
SwissProt (Mouse)
Q9DAK9
Available Sizes
Frequently Asked Questions
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