Rabbit anti-PGM1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PGM1
Synonyms
Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human PGM1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PGM1 expression in HepG2 (A), mouse heart (B), rat liver (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PGM1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5236
Gene ID (Mouse)
72157
SwissProt (Human)
P36871
SwissProt (Mouse)
Q9D0F9
SwissProt (Rat)
P38652
CAS Number
9007-83-4
Available Sizes
Curated Selection
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