Rabbit anti-PDPK1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PDPK1
Synonyms
PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1
Host
Rabbit
Reactivity
H, M, R, B, P, Rb, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDPK1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PDPK1 expression in Hela (A), PC12 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of PDPK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5170
Gene ID (Mouse)
18607
Gene ID (Rat)
81745
SwissProt (Human)
O15530
SwissProt (Mouse)
Q9Z2A0
SwissProt (Rat)
O55173
CAS Number
9007-83-4
Available Sizes
Curated Selection
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