Rabbit anti-PDC-E2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PDC-E2
CAS Number
9007-83-4
Synonyms
DLTA; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PBC; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human PDC-E2
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of PDC-E2 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D), rat kidney (E) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PDC-E2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1737
Gene ID (Mouse)
235339
Gene ID (Rat)
81654
SwissProt (Human)
P10515
SwissProt (Mouse)
Q8BMF4
SwissProt (Rat)
P08461
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