Rabbit anti-PCNA Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PCNA
Synonyms
Proliferating cell nuclear antigen; PCNA; Cyclin
Host
Rabbit
Reactivity
H, M, R, B, C, D, Mk, P, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PCNA expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5111
Gene ID (Mouse)
18538
Gene ID (Rat)
25737
SwissProt (Human)
P12004
SwissProt (Mouse)
P17918
SwissProt (Rat)
P04961
CAS Number
9007-83-4
Available Sizes
Curated Selection
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