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Rabbit anti-PCGF6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PCGF6

CAS Number

9007-83-4

Synonyms

MBLR; RNF134; Polycomb group RING finger protein 6; Mel18 and Bmi1-like RING finger; RING finger protein 134

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human PCGF6

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of PCGF6 expression in MCF7 (A), mouse liver (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PCGF6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

84108

Gene ID (Mouse)

71041

SwissProt (Human)

Q9BYE7

SwissProt (Mouse)

Q99NA9

Available Sizes

Frequently Asked Questions

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