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Rabbit anti-PCBD1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PCBD1

Synonyms

DCOH; PCBD; Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PCBD1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PCBD1 expression in SW620 (A), mouse kidney (B), mouse pancreas (C), rat liver (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PCBD1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5092

Gene ID (Mouse)

13180

Gene ID (Rat)

29700

SwissProt (Human)

P61457

SwissProt (Mouse)

P61458

SwissProt (Rat)

P61459

CAS Number

9007-83-4

Available Sizes

Curated Selection

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