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Anti-Mouse Sperm Protein sp56 Antibody

Mouse Monoclonal Antibody specific to Mouse Sperm Protein sp56

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Mouse Sperm Protein sp56 Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Mouse, Hamster

Immunogen

Mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient

Target

Mouse Sperm Protein sp56

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, IHC

Field of Research

Reproductive Biology

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

These antibodies may be used in <br><br>Immunohistochemistry on zinc-formaldehyde fixed, paraffin-embedded mouse testes to localize sp56 on mouse sperm and in immunoblots or ELISA to detect sp56. DILUTION INSTRUCTIONS

Storage Conditions

These antibodies are stable for at least on (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

These antibodies specifically recognize mouse sperm protein sp56. They will react with native or denatured, non-reduced sp56 but not with denatured, reduced sp56. These antibodies do not cross-react with human sperm. Relative antibody titers for antibody binding to solid phase sp56: 55101 = 66pM, 55102 = 4nM, 55103 = 29nM, 55104 = 70nM

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

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