Rabbit anti-Nucleostemin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Nucleostemin
Synonyms
E2IG3; NS; Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; NNP47; Nucleolar GTP-binding protein 3; Nucleostemin
Host
Rabbit
Reactivity
H
Immunogen
Recombinant full length protein of human Nucleostemin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Nucleostemin expression in HEK293T (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Nucleostemin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
26354
SwissProt (Human)
Q9BVP2
CAS Number
9007-83-4
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS9002185 Inquire
Rabbit anti-Escherichia coli O6:H1 (strain CFT073/700928/UPEC) ARNA Polyclonal Antibody
Ask
View Details 
MBS6348624-02 5x 0.1 mL
SLFN12, ID (SLFN12, Schlafen family member 12) (MaxLight 650)
Ask
View Details