Rabbit anti-Nucleolin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Nucleolin
Synonyms
Nucleolin; Protein C23
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Nucleolin
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Nucleolin expression in MCF7 (A), A549 (B), THP1 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Nucleolin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4691
Gene ID (Mouse)
17975
SwissProt (Human)
P19338
SwissProt (Mouse)
P09405
SwissProt (Rat)
P13383
CAS Number
9007-83-4
Available Sizes
Curated Selection
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