Rabbit anti-NIRF Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NIRF
Synonyms
NIRF; RNF107; E3 ubiquitin-protein ligase UHRF2; Np95/ICBP90-like RING finger protein; Np95-like RING finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; RING finger protein 107; Ubiquitin-like PHD and RING finger domain-containing protein 2; Ubiquitin-like-containing PHD and RING finger domains protein 2
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human NIRF
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of NIRF expression in Hela (A), PC3 (B), THP1 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of NIRF staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
115426
Gene ID (Mouse)
109113
SwissProt (Human)
Q96PU4
SwissProt (Mouse)
Q7TMI3
CAS Number
9007-83-4
Available Sizes
Curated Selection
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