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Rabbit anti-NIRF Antibody

Product Specifications

Background

Rabbit polyclonal antibody to NIRF

CAS Number

9007-83-4

Synonyms

NIRF; RNF107; E3 ubiquitin-protein ligase UHRF2; Np95/ICBP90-like RING finger protein; Np95-like RING finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; RING finger protein 107; Ubiquitin-like PHD and RING finger domain-containing protein 2; Ubiquitin-like-containing PHD and RING finger domains protein 2

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human NIRF

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of NIRF expression in Hela (A), PC3 (B), THP1 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of NIRF staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

115426

Gene ID (Mouse)

109113

SwissProt (Human)

Q96PU4

SwissProt (Mouse)

Q7TMI3

Available Sizes

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