Rabbit anti-NICE4 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NICE4
CAS Number
9007-83-4
Synonyms
KIAA0144; NICE4; Ubiquitin-associated protein 2-like; Protein NICE-4
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NICE4. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of NICE4 expression in MCF7 (A), SP2/0 (B), H9C2 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of NICE4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
9898
Gene ID (Mouse)
74383
SwissProt (Human)
Q14157
SwissProt (Mouse)
Q80X50
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
