Rabbit anti-NF-kappaB p65 (pT254) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to NF-kappaB p65 (pT254)

CAS Number

9007-83-4

Synonyms

NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Host

Rabbit

Reactivity

H, M, R, B, Mk, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65 (pT254) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of NF-kappaB p65 (pT254) expression in HeLa TNFa-treated (A), HL60 TNFa-treated (B) whole cell lysates.

IHC description

Immunohistochemical analysis of NF-kappaB p65 (pT254) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.