Rabbit anti-NET (pS357) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NET (pS357)
CAS Number
9007-83-4
Synonyms
NET; SAP2; ETS domain-containing protein Elk-3; ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2
Host
Rabbit
Reactivity
H, M, R, B, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NET (pS357) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of NET (pS357) expression in HUVEC PMA-treated (A), HT29 PMA-treated (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of NET (pS357) staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
2004
Gene ID (Mouse)
13713
SwissProt (Human)
P41970
SwissProt (Mouse)
P41971
Available Sizes
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