Rabbit anti-NEDD4 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NEDD4
Synonyms
KIAA0093; NEDD4-1; E3 ubiquitin-protein ligase NEDD4; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4
Host
Rabbit
Reactivity
H
Immunogen
Recombinant full length protein of human NEDD4
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of NEDD4 expression in Hela (A), Jurkat (B), A549 (C), SKOV3 (D) whole cell lysates.
IHC description
Immunohistochemical analysis of NEDD4 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4734
SwissProt (Human)
P46934
CAS Number
9007-83-4
Available Sizes
Curated Selection
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