Rabbit anti-NDUFS4 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NDUFS4
Synonyms
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; Complex I-18 kDa; CI-18 kDa; Complex I-AQDQ; CI-AQDQ; NADH-ubiquinone oxidoreductase 18 kDa subunit
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human NDUFS4
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of NDUFS4 expression in SW620 (A), MCF7 (B), HepG2 (C), mouse brain (D), rat liver (E) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of NDUFS4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4724
Gene ID (Mouse)
17993
Gene ID (Rat)
499529
SwissProt (Human)
O43181
SwissProt (Mouse)
Q9CXZ1
SwissProt (Rat)
Q5XIF3
CAS Number
9007-83-4
Available Sizes
Curated Selection
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