Rabbit anti-NCS1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NCS1
Synonyms
FLUP; FREQ; Neuronal calcium sensor 1; NCS-1; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human NCS1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of NCS1 expression in Hela (A), MCF7 (B), mouse bone marrow (C), rat kidney (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of NCS1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
23413
Gene ID (Mouse)
14299
Gene ID (Rat)
65153
SwissProt (Human)
P62166
SwissProt (Mouse)
Q8BNY6
SwissProt (Rat)
P62168
CAS Number
9007-83-4
Available Sizes
Curated Selection
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