Rabbit anti-Myosin Ic Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Myosin Ic
CAS Number
9007-83-4
Synonyms
Unconventional myosin-Ic; Myosin I beta; MMI-beta; MMIb
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Myosin Ic
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Rabbit
WB Description
Western blot analysis of Myosin Ic expression in HT29 (A), SKOV3 (B), mouse lung (C), rat heart (D) whole cell lysates.
IHC description
Immunohistochemical analysis of Myosin Ic staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Myosin Ic staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4641
Gene ID (Mouse)
17913
Gene ID (Rat)
65261
SwissProt (Human)
O00159
SwissProt (Mouse)
Q9WTI7
SwissProt (Rat)
Q63355
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC470352-500 1x 500 µL
CD31 / PECAM-1 (158-2B3), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC881231-100 1x 100 µL
Eosinophil Peroxidase (AHE-1), CF488A conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040158-100 1x 100 µL
Cytokeratin 17 (E3), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400391-500 1x 500 µL
CD81 / TAPA-1 (1.3.3.22), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC880147-500 1x 500 µL
Pgp9.5 (UCHL-1) (13C4), CF488A conjugate, 0.1mg/mL
Sign In for Pricing
View Details