Rabbit anti-Myoglobin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Myoglobin
Synonyms
Myoglobin
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Myoglobin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Myoglobin expression in HepG2 (A), mouse liver (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Myoglobin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4151
Gene ID (Mouse)
17189
Gene ID (Rat)
59108
SwissProt (Human)
P02144
SwissProt (Mouse)
P04247
SwissProt (Rat)
Q9QZ76
CAS Number
9007-83-4
Available Sizes
Curated Selection
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