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Rabbit anti-MsrA Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MsrA

CAS Number

9007-83-4

Synonyms

Mitochondrial peptide methionine sulfoxide reductase; Peptide-methionine (S) -S-oxide reductase; Peptide Met (O) reductase; Protein-methionine-S-oxide reductase; PMSR

Host

Rabbit

Reactivity

H

Immunogen

Recombinant full length protein of human MsrA

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of MsrA expression in HT29 (A), Hela (B), mouse heart (C), rat kidney (D), rat liver (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of MsrA staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

4482

SwissProt (Human)

Q9UJ68

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