Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-MMP13 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MMP13

Synonyms

Collagenase 3; Matrix metalloproteinase-13; MMP-13

Host

Rabbit

Reactivity

H, M, R, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MMP13. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of MMP13 expression in HeLa (A), SP2/0 (B), H9C2 (C), MCF7 (D), HT1080 (E), SCC4 (F) whole cell lysates.

IHC description

Immunohistochemical analysis of MMP13 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of MMP13 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

4322

Gene ID (Mouse)

17386

SwissProt (Human)

P45452

SwissProt (Mouse)

P33435

SwissProt (Rat)

P23097

CAS Number

9007-83-4

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Mouse POMGNT1 siRNA
abx929251-01 15 nmol

Mouse POMGNT1 siRNA

Ask
View Details
Mouse POMGNT1 siRNA
abx929251-02 30 nmol

Mouse POMGNT1 siRNA

Ask
View Details
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)
MBS1158274-01 0.02 mg (E-Coli)

Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)

Ask
View Details
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)
MBS1158274-02 0.1 mg (E-Coli)

Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)

Ask
View Details
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)
MBS1158274-03 0.02 mg (Yeast)

Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)

Ask
View Details
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)
MBS1158274-04 0.1 mg (Yeast)

Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDL185C-A (YDL185C-A)

Ask
View Details