Rabbit anti-MLKL Antibody
Product Specifications
Background
Rabbit polyclonal antibody to MLKL
Synonyms
Mixed lineage kinase domain-like protein; hMLKL
Host
Rabbit
Reactivity
H
Immunogen
Recombinant full length protein of human MLKL
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of MLKL expression in Hela (A), Jurkat (B), HT1080 (C), NCIH460 (D) whole cell lysates.
IHC description
Immunohistochemical analysis of MLKL staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
197259
SwissProt (Human)
Q8NB16
CAS Number
9007-83-4
Available Sizes
Curated Selection
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