Rabbit anti-MKK4 (pS257) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to MKK4 (pS257)
Synonyms
JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKK4. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of MKK4 (pS257) expression in K562 (A), Hela (B), MCF7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of MKK4 (pS257) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
6416
Gene ID (Mouse)
26398
SwissProt (Human)
P45985
SwissProt (Mouse)
P47809
CAS Number
9007-83-4
Available Sizes
Curated Selection
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