Rabbit anti-LIM Antibody
Product Specifications
Background
Rabbit polyclonal antibody to LIM
Synonyms
ENH; PDZ and LIM domain protein 5; Enigma homolog; Enigma-like PDZ and LIM domains protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human LIM
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of LIM expression in HepG2 (A), MCF7 (B), mouse lung (C), mouse kidney (D) whole cell lysates.
IHC description
Immunohistochemical analysis of LIM staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of LIM staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
10611
Gene ID (Mouse)
56376
Gene ID (Rat)
64353
SwissProt (Human)
Q96HC4
SwissProt (Mouse)
Q8CI51
SwissProt (Rat)
Q62920
CAS Number
9007-83-4
Available Sizes
Curated Selection
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