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Rabbit anti-LHX1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to LHX1

CAS Number

9007-83-4

Synonyms

LIM-1; LIM1; LIM/homeobox protein Lhx1; LIM homeobox protein 1; Homeobox protein Lim-1; hLim-1

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LHX1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of LHX1 expression in HEK293T (A), HepG2 (B), SP2/0 (C), H9C2 (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of LHX1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3975

Gene ID (Mouse)

16869

Gene ID (Rat)

257634

SwissProt (Human)

P48742

SwissProt (Mouse)

P63006

SwissProt (Rat)

P63007

Available Sizes

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