Rabbit anti-LCK (pY393) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to LCK (pY393)
Synonyms
Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Host
Rabbit
Reactivity
H, M, R, B, C, P, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LCK. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of LCK (pY393) expression in MCF7 TNFa-treated (A), mouse liver (B), rat liver (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of LCK (pY393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
3932
Gene ID (Mouse)
16818
Gene ID (Rat)
313050
SwissProt (Human)
P06239
SwissProt (Mouse)
P06240
SwissProt (Rat)
Q01621
CAS Number
9007-83-4
Available Sizes
Curated Selection
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