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Rabbit anti-LACI Antibody

Product Specifications

Background

Rabbit polyclonal antibody to LACI

Synonyms

LACI; TFPI1; Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human LACI

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of LACI expression in HEK293T (A), MCF7 (B), mouse lung (C), mouse heart (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of LACI staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

7035

Gene ID (Mouse)

21788

Gene ID (Rat)

29436

SwissProt (Human)

P10646

SwissProt (Mouse)

O54819

SwissProt (Rat)

Q02445

CAS Number

9007-83-4

Available Sizes

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