Rabbit anti-KLF1/5/7 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to KLF1/5/7
Synonyms
KLF1; EKLF; Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; KLF7; UKLF; Krueppel-like factor 7; Ubiquitous krueppel-like factor
Host
Rabbit
Reactivity
H, M, R, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human KLF1/5/7. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of KLF1/5/7 expression in Jurkat (A), VEC (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of KLF1/5/7 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
10661; 688; 8609
Gene ID (Mouse)
12224; 93691
SwissProt (Human)
Q13351; Q13887; O75840
SwissProt (Mouse)
P46099; Q9Z0Z7; Q99JB0
CAS Number
9007-83-4
Available Sizes
Curated Selection
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