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Rabbit anti-JNK3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to JNK3

CAS Number

9007-83-4

Synonyms

JNK3; JNK3A; PRKM10; SAPK1B; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JNK3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of JNK3 expression in NIH3T3 (A), SKNSH (B), HeLa (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of JNK3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5602

Gene ID (Mouse)

26414

Gene ID (Rat)

25272

SwissProt (Human)

P53779

SwissProt (Mouse)

Q61831

SwissProt (Rat)

P49187

Available Sizes

Curated Selection

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