Rabbit anti-JNK3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to JNK3
Synonyms
JNK3; JNK3A; PRKM10; SAPK1B; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human JNK3. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of JNK3 expression in NIH3T3 (A), SKNSH (B), HeLa (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of JNK3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5602
Gene ID (Mouse)
26414
Gene ID (Rat)
25272
SwissProt (Human)
P53779
SwissProt (Mouse)
Q61831
SwissProt (Rat)
P49187
CAS Number
9007-83-4
Available Sizes
Curated Selection
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