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Rabbit anti-IWS1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to IWS1

Synonyms

IWS1L; Protein IWS1 homolog; IWS1-like protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human IWS1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of IWS1 expression in NCIH460 (A), mouse lung (B), rat brain (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of IWS1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

55677

Gene ID (Mouse)

73473

Gene ID (Rat)

291705

SwissProt (Human)

Q96ST2

SwissProt (Mouse)

Q8C1D8

SwissProt (Rat)

Q3SWT4

CAS Number

9007-83-4

Available Sizes

Curated Selection

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