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Rabbit anti-ITPKA Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ITPKA

Synonyms

Inositol-trisphosphate 3-kinase A; Inositol 145-trisphosphate 3-kinase A; IP3 3-kinase A; IP3K A; InsP 3-kinase A

Host

Rabbit

Reactivity

H, M, R, B, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ITPKA. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1 mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ITPKA expression in A549 (A), LO2 (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of ITPKA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3706

Gene ID (Mouse)

228550

Gene ID (Rat)

81677

SwissProt (Human)

P23677

SwissProt (Mouse)

Q8R071

SwissProt (Rat)

P17105

CAS Number

9007-83-4

Available Sizes

Curated Selection

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