Rabbit anti-Islet-1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Islet-1
Synonyms
Insulin gene enhancer protein ISL-1; Islet-1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Islet-1
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Islet-1 expression in Hela (A), mouse thymus (B), rat thymus (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Islet-1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
3670
Gene ID (Mouse)
16392
Gene ID (Rat)
64444
SwissProt (Human)
P61371
SwissProt (Mouse)
P61372
SwissProt (Rat)
P61374
CAS Number
9007-83-4
Available Sizes
Curated Selection
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