Rabbit anti-iNOS Antibody
Product Specifications
Background
Rabbit polyclonal antibody to iNOS
Synonyms
NOS2A; Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human iNOS. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of iNOS expression in Jurkat (A), SHSY5Y (B), NIH3T3 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4843
Gene ID (Mouse)
18126
Gene ID (Rat)
24599
SwissProt (Human)
P35228
SwissProt (Mouse)
P29477
SwissProt (Rat)
Q06518
CAS Number
9007-83-4
Available Sizes
Curated Selection
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