Rabbit anti-IL-1RA Antibody
Product Specifications
Background
Rabbit polyclonal antibody to IL-1RA
Synonyms
IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human IL-1RA
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of IL-1RA expression in Hela (A), NIH3T3 (B), HL60 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of IL-1RA staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
3557
Gene ID (Mouse)
16181
Gene ID (Rat)
60582
SwissProt (Human)
P18510
SwissProt (Mouse)
P25085
SwissProt (Rat)
P25086
CAS Number
9007-83-4
Available Sizes
Curated Selection
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