Rabbit anti-IKK gamma (pS85) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to IKK gamma (pS85)
Synonyms
FIP3; NEMO; NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKK gamma. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of IKK gamma (pS85) expression in THP1 LPS-treated (A), Raw264.7 LPS-treated (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of IKK gamma (pS85) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
8517
Gene ID (Mouse)
16151
Gene ID (Rat)
309295
SwissProt (Human)
Q9Y6K9
SwissProt (Mouse)
O88522
SwissProt (Rat)
Q6TMG5
CAS Number
9007-83-4
Available Sizes
Curated Selection
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