Rabbit anti-IKK beta Antibody
Product Specifications
Background
Rabbit polyclonal antibody to IKK beta
Synonyms
IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
Host
Rabbit
Reactivity
H, M, R, B, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKK beta. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1 mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of IKK beta expression in HEK293T (A), human IKK beta full length protein (B) whole cell lysates.
IHC description
Immunohistochemical analysis of IKK beta staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3551
Gene ID (Mouse)
16150
Gene ID (Rat)
84351
SwissProt (Human)
O14920
SwissProt (Mouse)
O88351
SwissProt (Rat)
Q9QY78
CAS Number
9007-83-4
Available Sizes
Curated Selection
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