Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Rabbit anti-IKB epsilon Antibody

Product Specifications

Background

Rabbit polyclonal antibody to IKB epsilon

CAS Number

9007-83-4

Synonyms

IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon

Host

Rabbit

Reactivity

H, M, R, B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IKB epsilon. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of IKB epsilon expression in Jurkat (A), HeLa (B), PC12 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of IKB epsilon staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of IKB epsilon staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

4794

Gene ID (Mouse)

18037

SwissProt (Human)

O00221

SwissProt (Mouse)

O54910

Available Sizes

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

C1QTNF9-AS1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV720075 1.0 µg DNA

C1QTNF9-AS1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

Sign In for Pricing
View Details
LMO3 (LIM Domain only 3 (rhombotin-like 2), DAT1, MGC26081, RBTN3, RBTNL2, RHOM3, Rhom-3) (APC)
248231-APC 100 µL

LMO3 (LIM Domain only 3 (rhombotin-like 2), DAT1, MGC26081, RBTN3, RBTNL2, RHOM3, Rhom-3) (APC)

Sign In for Pricing
View Details
RT1-M5 Protein Vector (Rat) (pPB-C-His)
PV302878 500 ng

RT1-M5 Protein Vector (Rat) (pPB-C-His)

Sign In for Pricing
View Details
pGFP-C-shLenti shRNA Vector
TR30023 5 µg

pGFP-C-shLenti shRNA Vector

Sign In for Pricing
View Details
Rat Noradrenaline, NE ELISA Kit
CELI-66116r 96 Tests

Rat Noradrenaline, NE ELISA Kit

Sign In for Pricing
View Details
BAG1 Protein Vector (Rat) (pPM-C-His)
PV255761 500 ng

BAG1 Protein Vector (Rat) (pPM-C-His)

Sign In for Pricing
View Details